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Serial Dilution and Bacterial Plating Protocol
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Serial Dilutions & Bacteria Plating
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1. Use a clean, new transfer pipette to add 10mL of sterilized water to a 15mL culture tube. Label the tube "100" and according to its corresponding soil sample |
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2. Using the same pipette
to add 9mL of sterilized water to a second 15mL culture tube. Label the
tube "10-1" and according to its corresponding soil sample 3. Repeat step 2 two more times to two additional 15mL culture tubes, only label them "10-2" and "10-3" respectively |
3. Place 1cc of your soil sample into the "100" culture
tube; cap and shake vigorously
4. Using a new clean pipette, remove 1mL of the soil/water mixture from
the "100" tube and place into the "10-1" tube;
cap and shake vigorously
5. Using the same pipette as step 4, remove 1mL of the soil/water
mixture from the "10-1" tube and place into the "10-2"
tube; cap and shake vigorously
6. Using the same pipette as step 4, remove 1mL of the soil/water
mixture from the "10-2" tube and place into the "10-3"
tube; cap and shake vigorously
| 7. Plate 100µl
samples from each dilution onto their own seperate, labeled petri plates containing
nutrient agar -In our case, we used 3M Petrifilm Aerobic Count Bacteria Growth Plates and pressers to plate our bacteria  
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8. Allow to grow at room temperature for 48-72
hours
