Procedure
Materials |
||
Goggles
|
Gloves
|
Field Notebook
|
30 Nutrient Agars |
Aluminum Foil |
Conventional Oven |
Sharpie Marker
|
10 Flags
|
30 Ziploc Bags
|
Soil Core Extractor
|
Mallet
|
33 Pipets
|
50 Culture Tubes |
Chemical Test Kit |
Sterilized Water
|
Step – by – Step Instructions:
1. Find a location where there are observable moisture differences in the soil
2. Use observations to pick 5 different areas with different moisture levels located in the location found in step 1
* Take all soil samples at the same time on the same day*
3. Take one 15cm deep and 2cm wide soil sample using a soil extractor from each of the locations within the same time frame
4. Put samples in a labeled
plastic bag according to its location
*Be sure to perform steps 5 - 7 at the same time*
5. Perform serial dilutions on all
five samples
6. Plate 100 µl of the dilutions 10-2, 10-3, and 10-4, on bacteria nutrient agar plates
8. Construct oven-safe weigh boats and put the remaining soil in the weigh boat
9. Weigh the soil in grams and oven safe weigh boat and record the data
10. Allow soil to sit in a place
where sun shines for 24 hours
11. After soil sits for 24 hours, place in oven at 110°C and allow to bake in oven for 2½ hrs.
12. After 2½ hrs., allow the soil
samples to cool and then re-weigh the baked soil in grams
13. Determine the difference
between the soil samples before they were placed in the oven and afterwards
14. Record the data
15. Repeat steps 3-14 once a day for two more days